United States securities and exchange commission logo

                           March 17, 2023

       R. Erik Holmlin, Ph.D.
       President and Chief Executive Officer
       Bionano Genomics, Inc.
       9540 Towne Centre Drive, Suite 100
       San Diego, California 92121

                                                        Re: Bionano Genomics,
Statement on Form S-3
                                                            Filed March 10,
                                                            File No. 333-270459

       Dear R. Erik Holmlin:

              This is to advise you that we have not reviewed and will not
review your registration

               Please refer to Rules 460 and 461 regarding requests for
acceleration. We remind you
       that the company and its management are responsible for the accuracy and
adequacy of their
       disclosures, notwithstanding any review, comments, action or absence of
action by the staff.

                                                        Please contact Jane
Park at 202-551-7439 with any questions.


                           Division of Corporation Finance

                           Office of Industrial Applications and

       cc:                                              Phillip McGill, Esq.