United States securities and exchange commission logo July 29, 2022 Christopher Stewart Chief Financial Officer Bionano Genomics, Inc. 9540 Towne Centre Drive Suite 100 San Diego, CA 92121 Re: Bionano Genomics, Inc. Form 10-K for the Fiscal Year Ended December 31, 2021 Filed March 1, 2022 File No. 001-38613 Dear Mr. Stewart: We have completed our review of your filings. We remind you that the company and its management are responsible for the accuracy and adequacy of their disclosures, notwithstanding any review, comments, action or absence of action by the staff. Sincerely, Division of Corporation Finance Office of Life Sciences