March 16, 2020

R. Erik Holmlin, Ph.D.
President and Chief Executive Officer
Bionano Genomics, Inc.
9540 Towne Centre Drive, Suite 100
San Diego, California 92121

       Re: Bionano Genomics, Inc.
           Registration Statement on Form S-1
           Filed March 11, 2020
           File No. 333-237074

Dear Dr. Holmlin:

       This is to advise you that we have not reviewed and will not review your

        Please refer to Rules 460 and 461 regarding requests for acceleration.
We remind you
that the company and its management are responsible for the accuracy and
adequacy of their
disclosures, notwithstanding any review, comments, action or absence of action
by the staff.

       Please contact Jeffrey Gabor at 202-551-2544 with any questions.


                                                            Division of
Corporation Finance
                                                            Office of Life
cc:    James Pennington, Esq.