United States securities and exchange commission logo June 14, 2024 R. Erik Holmlin, Ph.D. Chief Executive Officer Bionano Genomics, Inc. 9540 Towne Centre Drive, Suite 100 San Diego, California 92121 Re: Bionano Genomics, Inc. Registration Statement on Form S-3 Filed June 10, 2024 File No. 333-280098 Dear R. Erik Holmlin: This is to advise you that we have not reviewed and will not review your registration statement. Please refer to Rules 460 and 461 regarding requests for acceleration. We remind you that the company and its management are responsible for the accuracy and adequacy of their disclosures, notwithstanding any review, comments, action or absence of action by the staff. Please contact Jane Park at 202-551-7439 with any questions. Sincerely, Division of Corporation Finance Office of Industrial Applications and Services cc: Marc Rivera, Esq.